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Description
Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell edge or interior (CIL 34929) of cytochalasin D-treated Xenopus fibroblasts stained with α-actinin primary antibody and 18-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal α-actinin at filament crossovers in the cell interior. A fluorescence image of fibroblast lamellipodia double stained with TRITC-phalloidin (red) and either α-actinin (green) is available at CIL 34924. The image corresponds to Figure 5f from J Cell Biol. 1999 May 31;145(5):1009-26. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Svitkina, Tatyana M.; Borisy, Gary G. (2021). CIL:34927, Xenopus laevis, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GH9GKS
Type
image
Identifier
ark:/20775/bb9290110m
Language
No linguistic content
Subject
Branching of actin filaments Cytochalasin D treatment Actin cytoskeleton Fibroblast Alpha actinin Cell edge Xenopus laevis Cell Image Library Group ID: 9875
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