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Description
Movie showing the dynamics of kinetchore microtubules during meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis that were experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 15 sec using a QImaging Retigo EXi CCD camera. Raw images were processed using 5-frame algorithm (Shribak and Oldenbourg, 2003). The time series used for this movie is included in this grouped set. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) LaFountain, James R.; Oldenbourg, Rudolf (2021). CIL:12373, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0XP743C
Type
image
Identifier
ark:/20775/bb95294411
Language
No linguistic content
Subject
Male meiosis chromosome segregation Nuclear chromosome Spindle Primary spermatocyte Nephrotoma suturalis Nephrotoma Cell Image Library Group ID: 3779
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