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Description
The adoption of cell-based therapies into the clinic will require tremendous large-scale expansion to satisfy future demand, and bioreactor-microcarrier cultures are best suited to meet this challenge. A robust optical imaging and image-analysis assay to non-destructively quantify cell number and cell volume was developed using fluorescence and elastic scattering contrast. This assay was successfully applied to monitor cellular growth of induced pluripotent derived mesenchymal stem cells (iH-MSCs) attached to spherical hydrogel microcarriers over time. Elastic scattering and fluorescence lightsheet microscopy were used to quantify cell volume and cell number at varying spatial scales. The fluorescence-based assay is used as ground truth data to correlate the elastic scattering-based assay data. Fig 1j Fixed Passage 4 day 7 ih-MSC on a gelMA microcarrier. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Benavides, Oscar R.; Gibbs, Holly C.; White, Berkley P.; Kaunas, Roland; Gregory, Carl A.; Walsh, Alex J.; Maitland, Kristen C. (2024). CIL: 54872. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0N016QM
Type
image
Identifier
ark:/20775/bb9807225p
Language
No linguistic content
Subject
Induced pluripotent stem cell-derived human mesenchymal stem cells Plasma membrane Nucleus Whole cell Cytoplasm Homo sapiens Cell Image Library Group ID: 20100
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