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Localization of MIIB/A (head, actin-binding domain of MIIA (myosin IIA), and tail domain of MIIB (myosin IIB)) in MIIA-deficient CHO.K1 cells. This Video corresponds to Fig. 10 B and video 6 from CB 183:543-554 , 2008. Images of GFP-MIIA/B (green) and paxillin-mOrange (magenta) were collected every 4 s using TIRF microscopy with 10 frames/s shown. Images were acquired in an inverted microscope (model IX70;Olympus). For dual GFP- mRFP/mCherry/mOrange, a dual-emission filter (z488/543) was used. Images were acquired with a charge-coupled device camera (Retiga Exi; Qimaging) and analyzed using MetaMorph software (MDS Analytical Technologies). Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Vicente-Manzanares, Miguel; Koach, Margaret A.; Whitmore, Leanna; Lamers, Marcelo L. (2021). CIL:12413, Cricetulus griseus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PG1QGT
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