Moving Image / CIL:19337, Balamuthia mandrillaris, cell by organism, eukaryotic cell, Eukaryotic Protist, Amoeboid Protist

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Title: CIL:19337, Balamuthia mandrillaris, cell by organism, eukaryotic cell, Eukaryotic Protist, Amoeboid Protist
Creator: Dunnebacke, Thelma
Contributor: Dunnebacke, Thelma
Date Created and/or Issued: 2021
Contributing Institution: UC San Diego, Research Data Curation Program
Collection: Cell Image Library
Rights Information: Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description: Balamuthia mandrillaris was isolated from a sample of soil from a plant in the home of a child who died of amebic encephalitis. These free living amoebae can be opportunistic pathogens, and in some cases have been diagnosed as the causative agent of the usually fatal amebic encephalitis. Other opportunistic pathogens are Naegleria and Acanthamoeba which feed by phagocytosis. Balamuthia feed by inserting the tips of their pseudopodia into cells. The other members of opportunistic pathogens, Naegleria and Acanthamoeba, are linked to this movie. The cells were grown in axenic media. Then they were photographed with a Canon Power Shot G3 with a setting at ISO 50 with an optical microlens at 4X and the quality set at RAW. The camera was attached to the phototube of the microscope with a 3X lens and photographed in phase contrast with a 100X oil immersion objective. Live amoebae were placed in a chamber prepared by inverting a coverslip with/or without cultured mammalian cells onto a 3X1 inch microscope slide with a thin rim of Vaseline; melted paraffin was brushed around the edge of the coverslip to seal it to the slide. Such cultures could be maintained for several days at room temperature. Further details are available in the attached publication.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Dunnebacke, Thelma (2021). CIL:19337, Balamuthia mandrillaris, cell by organism, eukaryotic cell, Eukaryotic Protist, Amoeboid Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0416W8N
Type: moving image
Identifier: ark:/20775/bb98707732
Language: No linguistic content
Subject: Pseudopodium assembly
Pseudopodium retraction
Pseudopodium organization
RP5
Amoeboid Protist
Cell
Pseudopodium
Eukaryotic cell
Eukaryotic Protist
Cell by organism
Balamuthia mandrillaris
Cell Image Library Group ID: 6503

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