Creative Commons Public Domain Dedication Constraint(s) on Use: This work may be used without prior permission. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Description
This is IMAGE #10 (second time-lapse set, 20 minutes) of a time lapse series contained with in this image group. It shows neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. The images were acquired with 10 minute intervals and show morphological plasticity of the dendritic arbor with branch additions and retractions. These cells were transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope. Images were acquired every 10 minutes for 1 hour. After 60 minutes, the animal was placed in a 50 microMolar anisomycin solution for ~1.5 hours. A second time lapse series (10 minute interval for 60 minutes) was then acquired. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Bestman, Jennifer; Cline, Holly (2022). CIL:36849, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0959HDZ
Type
image
Identifier
ark:/20775/bb99391461
Language
No linguistic content
Subject
Translation Morphogenesis of a branching structure Neuron development CNS neuron (sensu Vertebrata) Axon Cytoplasm Neuronal cell body Dendrite Xenopus laevis Cell Image Library Group ID: 9191
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