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Description
Distribution of interneurons expressing EGFP from the 5HT3 receptor promoter (Tg(Htr3a-EGFP)DH30Gsat, www.gensat.org) colabelled for calretinin in the cingulate cortex. A plexus of calretinin and EGFP positive axons can be seen in the superficial cortical layers. Many of the EGFP-expressing interneurons also express calretinin. Section (40 um) was imaged with a 32X, 0.4 NA Achroplan objective on a Zeiss Axiovert microscope. AF568 was excited at 562/40 nm and emission collected at 624/40 nm with Semrock filters and a single band dichroic (530-585 nm reflected, 601-800 nm transmitted). AF488 was excited at 482/35 nm and emission collected at 536/40 nm with Semrock filters and a single band dichroic (446-499 nm reflected, 513-735 nm transmitted). DAPI was excited at 350/50 nm and emission collected at 460/50 nm with a CLP400 dichroic (Chroma). Single images were captured for each channel using a Zeiss Axiocam MR CCD and pseudo-colored using Axiovision. See Davis and Puhl 2011 (Pubmed ID 21305052) for staining protocol. This image is part of a series characterizing EGFP expression from the 5HT3 promoter throughout the brain. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Davis, Margaret I. (2021). CIL:15005, Mus musculus, interneuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05M64VB
Type
Dataset
Language
No linguistic content; Not applicable
Subject
Cellular calcium ion homeostasis Cell communication by chemical coupling Serotonin receptor activity Axon Neuronal cell body Interneuron Mus musculus
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