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Description
Ema localizes to endosomes. Third instar larval garland cells expressing Ema-GFP fusion protein and stained for the multivesicular body ESCRT protein Hrs (red). Ema does not localize to MVB. Wandering third instar larvae were dissected in ice-cold PBS, and fixed with Bouin's solution for 5 min. Blocking and antibody incubation were performed in PBS containing 0.1% Triton X-100. Anti-HRS-FL (Lloyd et al., 2002) was used at 1:1,000. Cy3-conjugated secondary antibody (Jackson Immunoresearch Laboratories, Inc.) at 1:1,000. Confocal images were acquired with a confocal microscope (model C1; Nikon) and accompanying EZ-C1 software using argon (excitation at 488 nm) and HeNe (excitation at 543 and 633 nm) lasers and a 60x Plan-Apochromat NA 1.4 objective (Nikon) at room temperature. Samples for each experiment were processed using the same confocal gain setting. Image corresponds to Fig 3D in Kim et al. J Cell Biol. 188: 717-734. 2010. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Kim, Sungsu; Wairkar, Yogesh P.; Daniels, Richard W.; DiAntonio, Aaron (2021). CIL:13479, Drosophila melanogaster, garland cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0VQ31DC
Type
image
Identifier
ark:/20775/bb5501535r
Language
No linguistic content
Subject
Endocytosis Multivesicular body Endosome Garland cell Drosophila melanogaster Cell Image Library Group ID: 6022
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