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Moving Image / CIL:28779, Caenorhabditis elegans, embryo

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Title
CIL:28779, Caenorhabditis elegans, embryo
Creator
Desai, Arshad
Hyndman, Francie
Maddox, Paul S
Monen, Joost
Oegema, Karen
Contributor
Desai, Arshad
Hyndman, Francie
Maddox, Paul S.
Monen, Joost
Oegema, Karen
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 in which CeCENP-A has been depleted by RNAi in strain OD31. Diffuse nuclear GFP signal is evident during prophase. However, unlike the control (CIL 28778), no discrete GFP foci were seen until anaphase onset, at which point foci of GFP-KNL-2 formed along the spindle and on the chromatin. The video is ∼18 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Maddox, Paul S.; Hyndman, Francie; Monen, Joost; Oegema, Karen; Desai, Arshad (2021). CIL:28779, Caenorhabditis elegans, embryo. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MS3RDK
Type
moving image
Identifier
ark:/20775/bb5774693n
Language
No linguistic content
Subject
Prophase
Mitosis
Anaphase
CeCENP-A depletion
Embryo
Chromatin
Spindle
KNL-2
Caenorhabditis elegans
Cell Image Library Group ID: 11164

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