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Description
Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 in which CeCENP-A has been depleted by RNAi in strain OD31. Diffuse nuclear GFP signal is evident during prophase. However, unlike the control (CIL 28778), no discrete GFP foci were seen until anaphase onset, at which point foci of GFP-KNL-2 formed along the spindle and on the chromatin. The video is ∼18 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Maddox, Paul S.; Hyndman, Francie; Monen, Joost; Oegema, Karen; Desai, Arshad (2021). CIL:28779, Caenorhabditis elegans, embryo. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MS3RDK
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