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Description
To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, NIH3T3 cells were treated with scrambled siRNA (Scr) for 2 days, treated with DMSO for 30 min., and stained for βPIX (green) and vinculin (red). In scrambled siRNA-treated cells treated with DMSO, vinculin localized to large focal adhesions at the cell periphery that overlapped with βPIX. These findings help elucidate a functional link between MII and Rac1/Cdc42 GTPases, which may regulate protrusion/adhesion dynamics in migrating cells. This image is the original data file from Fig. 6A, “Requirement for βPIX in MII-regulated cell protrusion and adhesion.” J. Cell Biol. 2010. Vol. 190(4):663–674. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Lee, Chan-Soo; Choi, Chang-Ki; Shin, Eun-Young; Schwartz, Martin Alexander; Kim, Eung-Gook (2021). CIL:26541, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J08C9V3T J. Cell Biol. 2010. Vol. 190(4):663–674 https://www.ncbi.nlm.nih.gov/pubmed/?term=0713598
Type
Dataset
Language
No linguistic content; Not applicable
Subject
NIH/3T3 Regulation of cell migration Fibroblast Myosin II complex Actin filament Lamellipodium Mus musculus Cell Image Library Group ID: 8283
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