Creative Commons Public Domain Dedication Constraint(s) on Use: This work may be used without prior permission. Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description
Distribution of interneurons expressing EGFP from the 5HT3 receptor promoter (Tg(Htr3a-EGFP)DH30Gsat, www.gensat.org) colabelled for the CB1 cannabinoid receptor. This image is from the pyramidal cell layer in hippocampal CA1. Multiple EGFP+ bipolar interneurons are present within the pyramidal cell layer and a mesh of EGFP+ fibers and CB1+ axons is present around the pyramidal cell bodies, consistent with basket cell synapses. EGFP+ cells are also present in the stratum oriens and stratum radiatum. CB1 was detected with the L15 antibody (K. Mackie). Section (40 um) was imaged with a 32X, 0.4 NA Achroplan objective on a Zeiss Axiovert microscope. AF568 was excited at 562/40 nm and emission collected at 624/40 nm with Semrock filters and a single band dichroic (530-585 nm reflected, 601-800 nm transmitted). AF488 was excited at 482/35 nm and emission collected at 536/40 nm with Semrock filters and a single band dichroic (446-499 nm reflected, 513-735 nm transmitted). DAPI was excited at 350/50 nm and emission collected at 460/50 nm with a CLP400 dichroic (Chroma). Single images were captured for each channel using a Zeiss Axiocam MR CCD and pseudo-colored using Axiovision. See Davis and Puhl 2011 (Pubmed ID 21305052) for a detailed staining protocol. This image is part of a series characterizing EGFP expression from the 5HT3 promoter throughout the brain. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Davis, Margaret I. (2021). CIL:14969, Mus musculus, CNS interneuron, pyramidal cell, basket cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J07W6B91
Type
image
Identifier
ark:/20775/bb6901450c
Language
No linguistic content
Subject
Cell communication by chemical coupling Cannabinoid receptor activity Serotonin receptor activity Pyramidal cell Nucleus Dendrite Axon Basket cell Neuronal cell body CNS interneuron Mus musculus
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
