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Description
Sialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA). Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points. BFA was added according to the following protocol: Timepoint1: Before addition of BFA 0seconds (8 Z-slices at 1 um apart) Timepoint2: After addition of BFA 130seconds (8 Z-slices at 1 um apart) Timepoint3: 238 seconds (11 Z-slices at 1 um apart) Timepoint3 thru Timepoint48: Images captured every 5 seconds (11 Z-slices at 1 um apart at each time point) Images were collected on a Carl Zeiss spinning disk confocal with a 63X 1.4 NA objective with 250 ms exposures at 5 s intervals. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Sengupta, Prabuddha (2021). CIL:9159, Homo sapiens, permanent cell line cell, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QC02JR
Type
Dataset
Language
No linguistic content; Not applicable
Subject
HeLa ER to Golgi vesicle-mediated transport Permanent cell line cell Endoplasmic reticulum Golgi apparatus Epithelial cell Homo sapiens Cell Image Library Group ID: 7926
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