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Title
CIL:13448, Saccharomyces cerevisiae S288c
Creator
Bessman, Nicholas J
Burd, Christopher G
Ferguson, Kathryn M
Schmitz, Karl R
Setty, Thanuja Gangi
Wood, Christopher S
Contributor
Bessman, Nicholas J.
Burd, Christopher G.
Ferguson, Kathryn M.
Schmitz, Karl R.
Setty, Thanuja Gangi
Wood, Christopher S.
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Intracellular localization of GFP-GOLPH3 in S. cerevisiae frq1-1 temperature-conditional strain grown at restrictive temperature of 37C. In frq1-1 mutant after a 30 minute incubation at the restrictive temperature, GFP-GOLPH3 loses punctate localization and is mostly in the cytosol. The Golgi-localized PtdIns 4-kinase in yeast is composed of the Pik1 catalytic subunit and a myristoylated calcium-binding protein, Frq1. Vps74 is essential to maintain glycosyltransferases in the Golgi and results in this study reveal a previously unrecognized PtdIns4P-binding site in Vps74/GOLPH3 family proteins and link Pik1 signaling to retention of Golgi-resident proteins. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig1A GOLPH3/frq1-1 37C panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 1A include CIL#s 13439, 13440, 13441, 13442, 13443, 13444, 13445, 13446, 13447, 13448, 13458, 13459.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Wood, Christopher S.; Schmitz, Karl R.; Bessman, Nicholas J.; Setty, Thanuja Gangi; Ferguson, Kathryn M.; Burd, Christopher G. (2021). CIL:13448, Saccharomyces cerevisiae S288c. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04Q7SP2
Type
image
Identifier
ark:/20775/bb7583464q
Language
No linguistic content
Subject
Protein transport
Protein localization in Golgi apparatus
Retrograde vesicle-mediated transport, Golgi to ER
Frq1-1
Regulation of signal transduction
Phosphatidylinositol-4-phosphate binding
Calcium ion binding
Enzyme binding
Golgi membrane
Cytosol
Golgi apparatus
Nucleus
Extrinsic to membrane
Saccharomyces cerevisiae S288c
Cell Image Library Group ID: 6596

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