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Description
Frog (Xenopus laevis) egg arrested in metaphase of mitosis. Note the tiny metaphase spindle on the top left of the egg. Eggs were fixed in 90% MeOH; 10% (H20 500mM EGTA) and stained for DNA with YoPro1 (green) and tubulin (red) with antibody B-5-1-2 directly labeled with Alexa 647. A single z slice was collected with a Zeiss LSM 710 using a 10 X 0.3NA objective. Lasers were 488 (DNA stain) and 633 (Tubulin stain). Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Wuehr, Martin; Mitchison, Timothy J. (2021). CIL:9512, Xenopus laevis. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RJ4HN1
Type
image
Identifier
ark:/20775/bb7993542x
Language
No linguistic content
Subject
Meiotic metaphase II Spindle microtubule Condensed chromosome Xenopus laevis Cell Image Library Group ID: 7118
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