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Image / CIL:41357, Mus musculus, adenocarcinoma

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Title
CIL:41357, Mus musculus, adenocarcinoma
Creator
Guttman, Peter
Haymann, J. Bernard
Heim, Stefan
McNally, James G
Mueller, Florian
Muller, Waltraud G
Nagashima, Kunio
Rehbein, Stefan
Schneider, Gerd
Werner, Stephan
Contributor
Guttman, Peter
Haymann, J. Bernard
Heim, Stefan
McNally, James G.
Mueller, Florian
Muller, Waltraud G.
Nagashima, Kunio
Rehbein, Stefan
Schneider, Gerd
Werner, Stephan
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows a region of cytoplasm containing 2 lysosomes. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation),the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Muller, Waltraud G.; Haymann, J. Bernard; Nagashima, Kunio; Guttman, Peter; Werner, Stephan; Rehbein, Stefan; Schneider, Gerd; McNally, James G.; Heim, Stefan; Mueller, Florian (2021). CIL:41357, Mus musculus, adenocarcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W094Q0
Type
image
Identifier
ark:/20775/bb92560523
Language
No linguistic content
Subject
Lysosome organization
Cytoplasm organization
Cytoplasm
Lysosome
Adenocarcinoma
Mus musculus
Cell Image Library Group ID: 11959

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