Guttman, Peter Haymann, J. Bernard Heim, Stefan McNally, James G Mueller, Florian Muller, Waltraud G Nagashima, Kunio Rehbein, Stefan Schneider, Gerd Werner, Stephan
Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows a portion of the nucleus, with the inner and outer membranes of the nuclear envelope clearly resolved. The images have a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation),he latter corresponding to~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Muller, Waltraud G.; Haymann, J. Bernard; Nagashima, Kunio; Guttman, Peter; Werner, Stephan; Rehbein, Stefan; Schneider, Gerd; McNally, James G.; Heim, Stefan; Mueller, Florian (2021). CIL:41360, Mus musculus, adenocarcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00R9N8W
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
