Guttman, Peter Haymann, J. Bernard Heim, Stefan McNally, James G Mueller, Florian Muller, Waltraud G Nagashima, Kunio Rehbein, Stefan Schneider, Gerd Werner, Stephan
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Guttman, Peter Haymann, J. Bernard Heim, Stefan McNally, James G. Mueller, Florian Muller, Waltraud G. Nagashima, Kunio Rehbein, Stefan Schneider, Gerd Werner, Stephan
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Description
The image shows a portion of a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows the plasma membrane with cytoplasm above and medium below. Numerous vesicles are present in the cytoplasm. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Muller, Waltraud G.; Haymann, J. Bernard; Nagashima, Kunio; Guttman, Peter; Werner, Stephan; Rehbein, Stefan; Schneider, Gerd; McNally, James G.; Heim, Stefan; Mueller, Florian (2021). CIL:41367, Mus musculus, adenocarcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J07S7MK8
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