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Description
It has been shown that polygalacturonase (PGase) activity is induced in Agrobacterium uitis when inoculated to wounds on Vitis roots. Tissue maceration by PGase results in formation of root lesions. Initially, it appeared that this response was not obtained in Vitis stems, since no overt lesions were observed. In experiments reported here, inoculation of green Vitis stem sections withA. uitis also results in tissue browning and significantly greater electrolyte leakage compared to inoculation with water,A. tumefacietis biovars I and II, or anA. uitis strain (CG50) in which the PGase gene has been inactivated. A uitis inoculations of lignified stem sections produced browning in the carnbial region indicating possible PGase activity. A comparison of electrolyte leakage from A uitis inoculation of Vitis genotypes varying in crown gall susceptibility revealed that the highly susceptible cultivar Cabernet Sauvignon had significantly greater leakage than the more resistant Couderc 3309 and V. amurensis.
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