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Description
Mitochondrial distribution (visualized with Mitotracker CMXRos) was largely unaffected by partial fragmentation of the intermediate filament cytoskeleton, visualized here with vimentin antibody, after expression of a mutant form of the peripherin gene in a primary fibroblast. This primary culture derived from the mouse dorsal root ganglion was methanol-fixed at -20 degrees. Microscope - Zeiss Axioplan I Illumination - Mercury arc lamp Objective - 63X oil/1.4 NA Intermediate lens between obj and camera - 0.63X Camera - Axiocam MRM CCD Filters: 1. Ex 485/20 BP, Em 515-565 BP 2. Ex 560/40, Em 630/6 Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Parysek, Linda (2021). CIL:245, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04J0DDJ
Type
image
Identifier
ark:/20775/bb40341804
Language
No linguistic content
Subject
Cytoplasm organization ATP biosynthetic process Mitochondrion localization Intermediate filament-based process Mitochondrion Type III intermediate filament Fibroblast Mus musculus
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