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Description
Upon mitochondrial uncoupling, wild-type PINK1-YFP (green) and mCherry-Parkin (red) are localized to mitochondria in PINK1 knock-out mouse embryonic fibroblasts (PINK1 KO MEFs). Transfected PINK1 KO MEFs were treated with the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone) (10µM) for 3 hours. Live cell imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Supplemental Figure S1c, bottom left row of J Cell Biol, 191: 933-942, 2010. Images in Supplemental Figure S1c include CIL#s 13725, 13726, 13727, 13728. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13727, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TX3D3N J Cell Biol, 191: 933-942, 2010. https://www.ncbi.nlm.nih.gov/pubmed/?term=21115803
Type
Dataset
Language
No linguistic content; Not applicable
Subject
Ubiquitin-protein ligase activity Magnesium ion binding Calcium-dependent protein kinase activity Protein serine/threonine kinase activity Protein phosphorylation ATP binding Protein K63-linked ubiquitination Ubiquitin protein ligase binding C3HC4-type RING finger domain binding Oxidative phosphorylation uncoupler activity Intracellular protein kinase cascade Protein binding Zinc ion binding Mitochondrion degradation Protein ubiquitination involved in ubiquitin-dependent protein catabolic process Protein K48-linked ubiquitination Response to stress Cytosol Nucleus Mitochondrial inner membrane Mitochondrial outer membrane Fibroblast Mitochondrion Mus musculus Cell Image Library Group ID: 7051
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