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Description
Mitochondria in HeLa cells are identified by mito-YFP (green). When fixed cells are permeabilized with 0.25% Triton X-100 (TX-100), both antibodies to cyctochrome c (red), a mitochondrial matrix marker, and the antibody to Tom20 (white), a mitochondrial outer membrane marker, are able to bind their epitopes. Mito-YFP transfected HeLa cells were treated with DMSO for 3 hrs, fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100/PBS. Primary antibodies used were: anti-Cyt c mAb (BD) and anti-Tom20 mAb (BD); secondary antibodies used were: Alexa Fluor 594 and 647. Imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Fig 4b, 3rd row from top and is part of a differential permeabilization assay with and without the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-chlorophenyl hydrazone) that is further described in Fig 4b of J Cell Biol, 191: 933-942, 2010. Images in Fig 4 include CIL#s 13733, 13734, 13729, 13730, 13731, 13732, 13717, 13718, 13719, 13720, 13721, 13722, 13723, 13724. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13734, Homo sapiens, endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J03X85CQ
Type
image
Identifier
ark:/20775/bb48189561
Language
No linguistic content
Subject
Respiratory electron transport chain Heme binding HeLa Electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity P-P-bond-hydrolysis-driven protein transmembrane transporter activity Protein targeting to mitochondrion Cervical carcinoma Endothelial cell Mitochondrial outer membrane translocase complex Mitochondrion Mitochondrial intermembrane space Homo sapiens Cell Image Library Group ID: 7051
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