Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
YFP-Parkin (green) localizes to mitochondria (red) when HeLa cells are treated with the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone). HeLa cells stably expressing YFP-Parkin were transfected with control siRNA for 192 hours, treated with CCCP (10µM) for 3 hours and then stained with TMRE, a cationic fluorescent dye that is readily sequestered by active mitochondria. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). Image corresponds to Fig2d, middle panels in J Cell Biol. 191: 933-942, 2010. Images in Fig2d include CIL#s 13710, 13711, 13712. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13712, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WQ02H9 J Cell Biol. 191: 933-942, 2010. https://www.ncbi.nlm.nih.gov/pubmed/?term=21115803
Type
Dataset
Language
No linguistic content; Not applicable
Subject
Protein K63-linked ubiquitination Protein ubiquitination involved in ubiquitin-dependent protein catabolic process HeLa Zinc ion binding Protein binding Protein K48-linked ubiquitination Ubiquitin-protein ligase activity Mitochondrion degradation Oxidative phosphorylation uncoupler activity Epithelial cell Mitochondrion Nucleus Cervical carcinoma Cytosol Homo sapiens Cell Image Library Group ID: 7051
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
