Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Z-series images of mouse ES cells carrying DHFR BAC transgene array (clone 36) and differentiated into fibroblast-like cells. 3D FISH shows increase in number of lac operator foci (green) and change to a more chain / fiber like large-scale chromatin conformation for the BAC transgene array (red). DAPI staining is shown in blue. Lac operator sequences still segregate into foci but these are located within or peripheral to fiber-like segments visualized for the BAC FISH signal. Nonlinear folding of DNA sequences within linear large-scale chromatin "fiber" segments is implied by the appearance of these Lac operator repeat foci. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Sinclair, Paul; Bian, Qian; Plutz, Matt; Heard, Edith; Belmont, Andrew (2021). CIL:26567, Mus musculus, embryonic stem cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W957ZZ P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776 https://www.ncbi.nlm.nih.gov/pubmed/?term=20819934
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
