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Description
Mitochondria in HeLa cells are identified by mito-YFP (green) following treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-chlorophenyl hydrazone). When fixed cells are unpermeabilized (not treated with detergent), antibodies to cyctochrome c (red), a mitochondrial matrix marker, and Tom20 (white), a mitochondrial outer membrane marker, are unable to reach and bind their epitopes. Mito-YFP transfected HeLa cells were treated with CCCP (10µM) for 3 hrs, fixed with 4% paraformaldehyde and not permeabilized. Primary antibodies used were: anti-Cyt c mAb (BD) and anti-Tom20 mAb (BD); secondary antibodies used were: Alexa Fluor 594 and 647. Imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Fig 4b, 4th row from top and is a control experiment for a differential permeabilization assay with and without CCCP that is further described in Fig 4b of J Cell Biol, 191: 933-942, 2010. Images in Fig 4 include CIL#s 13733, 13734, 13729, 13730, 13731, 13732, 13717, 13718, 13719, 13720, 13721, 13722, 13723, 13724. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13730, Homo sapiens, endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0R21044
Type
image
Identifier
ark:/20775/bb0518571b
Language
No linguistic content
Subject
Protein targeting to mitochondrion HeLa Heme binding Electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity Cervical carcinoma Respiratory electron transport chain Oxidative phosphorylation uncoupler activity P-P-bond-hydrolysis-driven protein transmembrane transporter activity Mitochondrial outer membrane translocase complex Mitochondrion Mitochondrial intermembrane space Endothelial cell Homo sapiens Cell Image Library Group ID: 7051
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