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The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. After cultured in differentiation medium for 3 days, these cells differentiated into myotubes (Green) containing multiple nuclei (Blue). The C2C12 cells stably expressing FLAG-tagged rapamycin-resistant, kinase-inactive (RR/KI) mTOR were induced to differentiate in the presence of 50nM rapamycin (Rap) for 3 days. 0.2μg/ml follistatin was added to the medium for the last 2 days of differentiation. Follistatin was able to rescue the effect of Rap to allow myotube maturation. This image is one of different treatments in Figure 9B from JCB 189: 1157-1169, 2010. See also CIL: 13592, 13593, 13594. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Sun, Yuting; Ge, Yejing; Drnevich, Jenny; Zhao, Yong; Band, Mark; Chen, Jie (2021). CIL:13601, Mus musculus, myoblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0765D10
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