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Title
CIL:13717, Homo sapiens, endothelial cell
Creator
Jin, Seok Min
Kane, Lesley A
Lazarou, Michael
Narendra, Derek P
Wang, Chunxin
Youle, Richard J
Contributor
Jin, Seok Min
Kane, Lesley A.
Lazarou, Michael
Narendra, Derek P.
Wang, Chunxin
Youle, Richard J.
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
YFP-WT PINK1 (green) shows mitochondrial localization in HeLa cells following treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-chlorophenyl hydrazone). When fixed cells are unpermeabilized (not treated with detergent), antibodies to GFP (red) (used to identify YFP-WT PINK1), and Tom20 (white), a mitochondrial outer membrane marker, are unable to reach and bind their epitopes. YFP-WT PINK1 transfected HeLa cells were treated with CCCP (10µM) for 3 hrs, fixed with 4% paraformaldehyde and not permeabilized. Primary antibodies used were: anti-GFP polyclonal Ab (Invitrogen) and anti-Tom20 mAb (BD); secondary antibodies used were: Alexa Fluor 594 and 647. Imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Fig 4c, top row, and is a control experiment for a differential permeabilization assay to determine submitochondrial localization of PINK1 and that is further described in Fig 4c of J Cell Biol, 191: 933-942, 2010. Images in Fig 4 include CIL#s 13733, 13734, 13729, 13730, 13731, 13732, 13717, 13718, 13719, 13720, 13721, 13722, 13723, 13724.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13717, Homo sapiens, endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0XD10DQ
Type
image
Identifier
ark:/20775/bb1235301v
Language
No linguistic content
Subject
Intracellular protein kinase cascade
HeLa
Protein targeting to mitochondrion
Cervical carcinoma
Response to stress
Magnesium ion binding
P-P-bond-hydrolysis-driven protein transmembrane transporter activity
Protein phosphorylation
Oxidative phosphorylation uncoupler activity
ATP binding
Calcium-dependent protein kinase activity
C3HC4-type RING finger domain binding
Protein serine/threonine kinase activity
Ubiquitin protein ligase binding
Mitochondrial outer membrane
Endothelial cell
Mitochondrial outer membrane translocase complex
Mitochondrion
Mitochondrial inner membrane
Homo sapiens
Cell Image Library Group ID: 7051

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