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Description
PINK1-YFP R98F (green) is found localized to mitochondria (red) in the presence of the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone). The R98F mutation in PINK1-YFP partially inhibits proteolytic cleavage. HeLa cells expressing PINK1-YFP R98F were stained with 10nM MitoTracker Red for 30 min to see mitochondrial morphology and then treated with 10µM CCCP for 3 hours. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). This image corresponds to Fig 3d, R98F/CCCP bottom panels in J Cell Biol. 191: 933-942, 2010. Images in Fig3d include CIL#s 13713, 13714, 13715, 13716. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13714, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QF8RK6
Type
image
Identifier
ark:/20775/bb30100621
Language
No linguistic content
Subject
ATP binding Oxidative phosphorylation uncoupler activity Intracellular protein kinase cascade Protein phosphorylation HeLa C3HC4-type RING finger domain binding Response to stress Protein serine/threonine kinase activity Magnesium ion binding Calcium-dependent protein kinase activity Ubiquitin protein ligase binding Epithelial cell Mitochondrion Mitochondrial outer membrane Mitochondrial inner membrane Cervical carcinoma Homo sapiens Cell Image Library Group ID: 7051
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