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Description
YFP-Parkin (green) localizes to mitochondria (red) when HeLa cells are transfected with control siRNA (siCtrl) and treated with the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone). HeLa cells stably expressing YFP-Parkin were transfected with control siRNA for 192 hours, treated with CCCP (10µM) for 1 hour and then stained with TMRE, a cationic fluorescent dye that is readily sequestered by active mitochondria. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). Image corresponds to Fig1f, middle panels and is a control for other images in Fig1f in J Cell Biol. 191: 933-942, 2010. Images in Fig1f include CIL#s 13707, 13708, 13709. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13707, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0F18XFV
Type
image
Identifier
ark:/20775/bb3419622w
Language
No linguistic content
Subject
Zinc ion binding Protein K48-linked ubiquitination Oxidative phosphorylation uncoupler activity Mitochondrion degradation Protein K63-linked ubiquitination Protein binding Ubiquitin-protein ligase activity HeLa Protein ubiquitination involved in ubiquitin-dependent protein catabolic process Epithelial cell Mitochondrion Cervical carcinoma Cytosol Nucleus Homo sapiens Cell Image Library Group ID: 7051
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