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Description
Expression of R98F PINK1-YFP (green) induces mitochondrial translocation of mCherry-Parkin (red) in the presence of the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone), indicating that this PINK1 mutant is functional for inducing Parkin translocation when located on the outer michondrial membrane. The R98F mutation in PINK1-YFP partially inhibits proteolytic cleavage. Transfected HeLa cells were treated with CCCP (10 µM) for 1 hr. Live cell imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Fig 4e, bottom row, with DMSO control in Fig 4e, top row of J Cell Biol, 191: 933-942, 2010. Images in Fig 4 include CIL#s 13733, 13734, 13729, 13730, 13731, 13732, 13717, 13718, 13719, 13720, 13721, 13722, 13723, 13724. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13724, Homo sapiens, endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZP44VD
Type
image
Identifier
ark:/20775/bb3760924g
Language
No linguistic content
Subject
Protein binding ATP binding Response to stress Protein K48-linked ubiquitination Cervical carcinoma Ubiquitin-protein ligase activity Zinc ion binding Mitochondrion degradation Ubiquitin protein ligase binding HeLa C3HC4-type RING finger domain binding Protein serine/threonine kinase activity Intracellular protein kinase cascade Protein phosphorylation Oxidative phosphorylation uncoupler activity Calcium-dependent protein kinase activity Protein ubiquitination involved in ubiquitin-dependent protein catabolic process Protein K63-linked ubiquitination Magnesium ion binding Mitochondrion Mitochondrial outer membrane Cytosol Nucleus Mitochondrial inner membrane Endothelial cell Homo sapiens Cell Image Library Group ID: 7051
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