Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
YFP-Parkin (green) does not translocate to mitochondria (red) when HeLa cells are transfected with control siRNA (siCtrl). HeLa cells stably expressing YFP-Parkin were transfected with control siRNA for 192 hours, treated with DMSO for 1 hour and then stained with TMRE, a cationic fluorescent dye that is readily sequestered by active mitochondria. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). Image corresponds to Fig1f, top panels and is a control for other images in Fig1f in J Cell Biol. 191: 933-942, 2010. Images in Fig1f include CIL#s 13707, 13708, 13709. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13708, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0P55M6D
Type
image
Identifier
ark:/20775/bb65937115
Language
No linguistic content
Subject
Protein K48-linked ubiquitination Protein ubiquitination involved in ubiquitin-dependent protein catabolic process Protein K63-linked ubiquitination Zinc ion binding HeLa Protein binding Ubiquitin-protein ligase activity Mitochondrion degradation Cervical carcinoma Mitochondrion Cytosol Nucleus Epithelial cell Homo sapiens Cell Image Library Group ID: 7051
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
