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Description
Upon mitochondrial uncoupling, wild-type PINK1-YFP (green) is localized in mitochondria (red) in HeLa cells. HeLa cells expressing WT PINK1-YFP were stained with 10nM MitoTracker Red (red) for 30 min to see mitochondrial morphology prior to treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone) (10µM) for 3 hours. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). This image corresponds to Fig 3d, WT/CCCP top panels in J Cell Biol. 191: 933-942, 2010. Images in Fig3d include CIL#s 13713, 13714, 13715, 13716. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13713, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0J38R85
Type
image
Identifier
ark:/20775/bb6969141n
Language
No linguistic content
Subject
ATP binding C3HC4-type RING finger domain binding Oxidative phosphorylation uncoupler activity Response to stress HeLa Intracellular protein kinase cascade Protein serine/threonine kinase activity Protein phosphorylation Ubiquitin protein ligase binding Calcium-dependent protein kinase activity Magnesium ion binding Mitochondrial outer membrane Cervical carcinoma Epithelial cell Mitochondrial inner membrane Mitochondrion Homo sapiens Cell Image Library Group ID: 7051
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