Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Upon mitochondrial uncoupling, wild-type PINK1-YFP (green) and mCherry-Parkin (red) are localized to mitochondria in PINK1 knock-out mouse embryonic fibroblasts (PINK1 KO MEFs). Transfected PINK1 KO MEFs were treated with the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone) (10µM) for 3 hours. Live cell imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Supplemental Figure S1c, bottom left row of J Cell Biol, 191: 933-942, 2010. Images in Supplemental Figure S1c include CIL#s 13725, 13726, 13727, 13728. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13727, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TX3D3N
Type
image
Identifier
ark:/20775/bb7139796r
Language
No linguistic content
Subject
Ubiquitin-protein ligase activity Magnesium ion binding Calcium-dependent protein kinase activity Protein serine/threonine kinase activity Protein phosphorylation ATP binding Protein K63-linked ubiquitination Ubiquitin protein ligase binding C3HC4-type RING finger domain binding Oxidative phosphorylation uncoupler activity Intracellular protein kinase cascade Protein binding Zinc ion binding Mitochondrion degradation Protein ubiquitination involved in ubiquitin-dependent protein catabolic process Protein K48-linked ubiquitination Response to stress Cytosol Nucleus Mitochondrial inner membrane Mitochondrial outer membrane Fibroblast Mitochondrion Mus musculus Cell Image Library Group ID: 7051
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
