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Image / CIL:13715, Homo sapiens, epithelial cell, cervical carcinoma

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Title
CIL:13715, Homo sapiens, epithelial cell, cervical carcinoma
Creator
Jin, Seok Min
Kane, Lesley A
Lazarou, Michael
Narendra, Derek P
Wang, Chunxin
Youle, Richard J
Contributor
Jin, Seok Min
Kane, Lesley A.
Lazarou, Michael
Narendra, Derek P.
Wang, Chunxin
Youle, Richard J.
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
PINK1-YFP R98F (green) is found localized to mitochondria (red) in the absence of the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone). The R98F mutation in PINK1-YFP partially inhibits proteolytic cleavage. HeLa cells expressing PINK1-YFP R98F were stained with 10nM MitoTracker Red for 30 min to see mitochondrial morphology and then treated with DMSO for 3 hours. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). This image corresponds to Fig 3d, R98F/DMSO bottom panels in J Cell Biol. 191: 933-942, 2010. Images in Fig3d include CIL#s 13713, 13714, 13715, 13716.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13715, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00R9N3N
Type
image
Identifier
ark:/20775/bb90852055
Language
No linguistic content
Subject
HeLa
Ubiquitin protein ligase binding
Intracellular protein kinase cascade
Response to stress
ATP binding
C3HC4-type RING finger domain binding
Protein serine/threonine kinase activity
Protein phosphorylation
Magnesium ion binding
Calcium-dependent protein kinase activity
Mitochondrion
Mitochondrial outer membrane
Mitochondrial inner membrane
Epithelial cell
Cervical carcinoma
Homo sapiens
Cell Image Library Group ID: 7051

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